Recent advances in long-read sequencing techniques have produced large amounts of high-quality rRNA marker gene data about eukaryotic organisms, but many of these taxa have remained unknown at the highest taxonomic levels: phylum and kingdom. Now, via a thorough analysis of the EUKARYOME long-read database, an international team led by Prof. Leho Tedersoo (University of Tartu, Estonia) has discovered that a large proportion of the unknown eukaryotes belong to deep, hitherto undescribed fungal lineages.