Nature Biotechnology, Published online: 26 June 2026; doi:10.1038/s41587-026-03206-0

Spatial-EV-seq is a method for spatially resolved profiling of extracellular vesicles (EVs) in tissues while preserving EVs’ native distribution. By integrating EV mapping with spatial transcriptomics, Spatial-EV-seq enables location-specific analysis of EVs and their communication networks within the tissue microenvironment, establishing a new paradigm in the field of spatial EV biology.